HISTOGENEX NV
DRIE EIKENSTRAAT 661
B-2650 EDEGEM
BELGIUM
TEL: +32-(0)3-2804815
FAX: +32-(0)3-2804861

 
 

  • IMMUNOHISTOCHEMISTRY

  • DETECTION OF APOPTOSIS

  • CYTOLOGY

  • CONFOCAL MICROSCOPY

  • FLUORESCENCE MICROSCOPY

  • PLASTIC EMBEDDING

  • IN SITU HYBRIDIZATION

  • LASER CAPTURE MICRODISSECTION

  • CO-LOCALIZATION

  • ELECTRON MICROSCOPY

HistoGeneX provides complete processing of tissue samples from laboratory animals to human biopsies for routine morphological research and cytology

HistoGeneX offers services for advanced research using a specialized developed processing system in which both histological and molecular information are optimally preserved

  • IMMUNOHISTOCHEMISTRY

  • LIQUID-BASED CYTOLOGY

  • LCM ON PARAFFIN EMBEDDED SECTIONS

  • IMMUNO-STAINING ON PLASTIC EMBEDDED MATERIAL
IMMUNOHISTOCHEMISTRY
_________________________________
HistoGeneX offers comprehensive immunohistochemical services using a battery of methods to preserve antigenicity of proteins during tissue processing or by using antigen retrieval techniques on sections
SC-35 stain
in myocytes of
dilated cardiomyopathy
HistoGeneX has more than 250 validated immunohistochemical staining assays based on monoclonal and polyclonal antibodies against human, mice, rabbit and pig epitopes
New antibodies are continuously under investigation to detect new molecular targets on tissue sections
HISTOLOGICAL IMAGES
MORE
PICTURES IN OUR
GALLERY
Caspase-14 in normal human skin
CD68 (blue) with Aß42 (red) in human Alzheimer brain
GFAP (blue) with Aß42 (red) in human Alzheimer brain
DETECTION OF APOPTOSIS
_________________________________
Prorammed cell death plays an important role in controlling cell number during development and throughout an organism's life by removal of cells at the appropriate time. However, programmed cell death is not confined to apoptosis only since other programmed cell death types are involved in the cell homeostasis. Therefore, detection of apoptosis by the TUNEL technique should always be combined with additional techniques (activated caspase-3, morphological criteria)

DETECTION OF
APOPTOSIS BY
THE TUNEL TECHNIQUE IS COMBINED WITH ADDITIONAL ASSAYS
Detection of TUNEL labeling in cell culture
Detection of cleaved Caspase-3 in cell culture
Detection of cleaved PARP in cell culture
CYTOLOGY _________________________________
HistoGeneX performs liquid based thin cell preparation for screening and detection of cervical cancer, pre-cancerous lesions, atypical cells for clinical trials
The Auto Prep system for preparing the liquid based suspension
The Autocyte system for preparing and staining liquid based thin cell preparation
PAP Class I
PAP Class II
All cytologic categories of cervical cancer are scored using the Bethesda System and PAP classification
CONFOCAL MICROSCOPY _________________________________
3D-RECONSTRUCTION
BY
CONFOCAL MICROSCOPY
Confocal Scanning Microscopy permits fluorescent visualization in a single plane to avoid out of focus information
At HistogeneX high quality confocal microscopy can be performed
3D reconstruction of
an ICC in Guinea Pig
stomach
Confocal image of living
tissue of the Guinea Pig ileum
FLUORESCENCE MICROSCOPY _________________________________

A powerful technique for localizing multiple organelles and epitopes in cells and tissues is fluorescent microscopy

HistoGeneX has expertise in stainings with multiple fluorescent labels which are detected by Axioplan 2 and Olympus fluorescence microscopes

Mitotracker in cell culture
(nucleus in blue)
Cytochrome-C stain in cell culture
(nucleus in blue)
Combination of Mitotracker and Cytochrome-C
(nucleus in blue)
PLASTIC EMBEDDING _________________________________

HistoGeneX has considerable experience with plastic embeddings

Plastic embedding is used for tissues, like stented arteries or bone, that are difficult to process by conventional paraffin embedding
Stented artery embedded in plastic
NEW AT HISTOGENEX

HistogeneX provides immunohistochemistry of nuclear epitopes on plastic embedded material

 
Image of a Plastic Embedded stented artery
RAM-11 stain for detection of the inflammatory response after stent placement
KI-67 stain for detection of cell proliferation after stent placement
Combination of TUNEL and SC35 to study apoptosis in stent restenosis
LASER CAPTURE MICRODISSECTION _________________________________

Laser Capture Microdissection is an exciting tool for collecting target cells from tissue sections overcoming the heterogeneity of tissues

At HistogeneX Laser Capture Microdissection is used in combination with RNA, DNA or proteins analysis

 
Focal areas with a diameter of 5 µm can be captured from tissue sections by laser Capture Microdissection. The captured cells are further processed for RNA, DNA or protein analysis
At HistoGeneX LCM can be performed on frozen as well as on paraffin embedded tissue without loosing molecular information
Hippocampus of a rat brain before LCM
Hippocampus of a rat brain after LCM
CO-LOCALIZATION _________________________________

Co-localization of both cytoplasmic and nuclear epitopes can be done by a stain and washout technique developed at HistoGeneX

A powerfull method to co-localize different nuclear epitopes
CO-LOCALIZATION
FOR
NUCLEAR EPITOPES
TUNEL labeling in heart tissue Overlay shows SC35 stain at the same tissue section
(Cardiovasc Res)
TUNEL labeling in atherosclerotic plaque
Overlay shows SC35 stain at the same tissue section
(Am J Pathol)
HistoGeneX NV, June 2002